ACADEMIC ACHIEVEMENTS

National Merit Scholarship Scheme 2006 (Rank 72 in Madhyamik Examination)

Scheme of Scholarship for College and University Students, Government of India, Ministry of Human Resource and Development, Department of Higher Education 2008.

Order of Merit in B.Sc. Examination from University of Calcutta 2011 (Rank 5)

Order of Merit in M.Sc. Examination from University of Calcutta 2013 (Rank 5; Internal)

CSIR - NET RANKINGS

CSIR-NET June 2014, LS- 044: ROLL NO.: 350799

CSIR-NET December 2013, LS- 054: ROLL NO.: 351903

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PROJECTS UNDERTAKEN

miR-200a prevents renal fibrogenesis through repression of TGF-β2 expression (Department of Zoology, University of Calcutta).

Investigating the role of Tbx20 in Cardiomyocyte protection post cardiac injury (Funded by FRPDF, Department of Life Sciences, Presidency University).

Tbx20 functions in epicardial cell proliferation and differentiation (Funded by UGC-Start Up, Department of Life Sciences, Presidency University).

FoxO transcription factor function in Cardiomyocyte proliferation (Funded by DBT India, Department of Life Sciences and Biotechnology, Jadavpur University).

Asporin function in aortic endothelial cell mineralization and calcification (Funded by UGC-Major, Department of Life Sciences, Presidency University).

Tbx20 function in cardiomyocyte proliferation and survival following cardiac injury (Funded by DBT India, Department of Life Sciences, Presidency University and Department of Life Sciences and Biotechnology, Jadavpur University).

Adamts4 as a marker of cardiac stress (Funded by DST, Department of Life Sciences and Biotechnology, Jadavpur University).

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CONFERENCE/SEMINARS ATTENDED

Participation at the National Symposium entitled “Modern Trends in Biological Sciences” organized by the Physiological Institute Presidency University held on 21st February, 2014.

Poster presentation in the XXXVIII All India Cell Biology Conference and International Symposium on “Cellular Response to Drugs” held at CSIR-CDRI, Lucknow during December 10-12, 2014.

Part of organizing committee and poster presentation in the 1st National Symposium on “Molecules to Systems” held at Department of Biological Science, Presidency University during February 29 -31, 2015.

Poster presentation in the XXXIX All India Cell Biology Conference and International Symposium on “Cellular Organization and Dynamics” held at Thiruvananthapuram, Kerala during December 6-8, 2015.

Part of organizing committee of the West Bengal State Science Congress held at Presidency University during February, 2016..

PUBLICATIONS

Paper Title : Tbx20 promotes H9c2 cell survival against oxidative stress and hypoxia in vitro

Name of the journal : Molecular and Cellular Biochemistry (IF: 2.613; 2016)

Authorship status : Peer Review

Paper Title : β-catenin stabilization promotes proliferation and increase in Cardiomyocyte number in avian embryonic epicardial explant culture

Name of the Journal : Journal of Biosciences (IF: 1.419; 2015)

Authorship status : Peer Review

SKILL SET

SOFTWARE SKILLS

Proficient in Windows 2007, 2013 & XP

Proficient in MS – OFFICE 2000, 2003 & 2007 (Word, Excel, PowerPoint, Outlook)

Proficient in Corel Draw; Adobe Photoshop
Internet Proficient

MICROBIOLOGICAL TECHNIQUES

Basic microbiology technique (preparation of culture media, plating methods).

Competent cell preparation

TECHNICAL SKILLS
CELL CULTURE TECHNIQUES

Animal Cell Cultures: Secondary Culture (H9c2; HEK293), Primary Culture (Early Embryonic Chick: Pro-epicardial organ, Epicardium; Late Embryonic Chick: Cardiomyocyte, Fibroblast; Adult Chick: Valvular cells.)

Culture Techniques: Cell maintenance, stress induction; Transfection (Gain-of-Function); siRNA (Loss-of-Function); Cell counting; Drug delivery; Cell freezing; Cell revival.

HISTOLOGY

Tissue fixation; Embedding; Sectioning; Staining.

STATISTICAL DATA INTERPRETATION

Student’s t-test; ANOVA

MOLECULAR TECHNIQUES

Transcript Level: RNA Isolation; DNA isolation; cDNA synthesis; Reverse Transcriptase PCR; Realtime PCR; Gel Electrophoresis; Insitu-Hybridisation.

Protein Level: Protein extraction; Protein estimation; SDS-PAGE; Western Blot; Antibody Staining.
Others: Cloning; Plasmid Isolation and Elusion; RFLP

INSTRUMENTATION

Cell culture Instruments: Biosafety Laminar Hood; CO2 Incubator; Flow-cytometer; Haemocytometer; Temperature Control Water-bath; Steri-pippette and micro-pipette and Liquid Nitrogen handling.

Spectros: Spectrophotometer; Fluorescence spectrometer; UV spectrometer.

Microscopy: Bright field Microscope; Inverted Microscope; Fluorescence Upright Microscope; Fluorescence Inverted Microscope.

Centrifuge: Bench top centrifuge; Cooling Centrifuge; Ultra Centrifuge.

PCR: Normal thermal cycler; Real-time PCR.

Others: UV Trans-illuminator; Gel-doc; ELISA plate reader; Homogenizer; Vortex; Rocker; pH meter; Gel electrophoresis and SDS-PAGE casting and running apparatus.

Freezer: 4◦C; -20◦C; -80◦C.

TRAININGS UNDERTAKEN

Puja Sen

Short term training in handling Drosophila, maintaining culture assisting in a project of Visual observation detailing in Drosophila imaginal discs as a part of M.Sc. programme.

Puja Sen

Short term training on molecular techniques (6months) at National Institute of Cholera and Enteric diseases (NICED) Kolkata as a part of M.Sc. programme.

Puja Sen

Short term training on handling laboratory mouse for clinical studies.

Contact Us

332N Agarwala Garden Road, PO-Sahapur, Kolkata – 700038, West Bengal, India

Phone + 91 7278815371 / 91 8100633804

Email sen.puja89@gmail.com

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